An improved method for purifying bacterial genomic DNAs for direct sequencing by capillary automated sequencer
نویسندگان
چکیده
منابع مشابه
Footprinting with an automated capillary DNA sequencer.
Footprinting is a valuable tool for studying DNA-protein contacts. However, it usually involves expensive, tedious and hazardous steps such as radioactive labeling and analyses on polyacrylamide sequencing gels. We have developed an easy four-step footprinting method involving (i) the generation and purification of a PCR fragment that is fluorescently labeled at one end with 6-carboxyfluorescei...
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ژورنال
عنوان ژورنال: Technical Tips Online
سال: 2001
ISSN: 1366-2120
DOI: 10.1016/s1366-2120(08)70160-0